PROFIL ASAM AMINO DAN ASAM LEMAK DALAM FERMENTASI INASUA GURARA
Abstract
Pengolahan inasua yang dilakukan masyarakat Teon, Nila, dan Serua di Maluku terdiri atas inasua tanpa nira kelapa dan inasua nira kelapa. Kedua produk fermentasi ini memiliki karakteristik sensorik dan masa simpan yang berbeda. Karakteristik sensorik bahan pangan sangat ditentukan oleh kadar asam amino dan asam lemak dalam pangan tersebut. Penelitian ini bertujuan untuk mengkarakterisasi profil asam amino dan asam lemak dalam kedua jenis inasua. Sampel inasua menggunakan ikan gurara (Lutjanus vitta) sebagai bahan dasar yang diambil dari pengrajin di Desa Layeni, Maluku Tengah. Analisis kadar asam amino menggunakan High Performance Liquid Chromatography (HPLC), sedangkan kadar asam lemak menggunakan Gas Chromatography (GC). Hasil penelitian menunjukkan bahwa kadar asam amino dan asam lemak meningkat sejalan bertambahnya waktu fermentasi. Kadar asam amino total inasua tanpa nira adalah 20,96 %, sedangkan inasua nira hanya mencapai 15,31%. Kadar asam lemak total inasua nira adalah 87,48 %, sedangkan inasua tanpa nira hanya mencapai 58,59 %. Asam amino tertinggi pada kedua inasua adalah asam glutamat, sedangkan asam lemak tertinggi adalah asam palmitat. Profil asam amino dan asam lemak diperlukan sebagai referensi untuk memperbaiki nilai nutrisi dan cita rasa inasua sebagai salah satu produk pangan lokal di Maluku.
Kata kunci : Asam glutamat, asam palmitat, karakteristik sensorik, nira kelapa
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