2654-5292
PENINGKATAN KANDUNGAN ASAM AMINO HIDROLISAT TEMPE SEMANGIT MELALUI PENGGUNAAN ENZIM PROTEASE DARI DAUN KELOR
Abstract
Longer time of fermentation treatment in overripe tempeh causes an increase in amino acid content in overripe tempe. The addition of protease enzymes from dial oleifera leaves to overripe tempeh will cause protein hydrolysis that leads to an increase in amino acid content..In this study, hydrolysate from overripe tempeh was made with different fermentation time variation for 2 days, 3 days, 4 days and 5 days and the addition of protease enzyme from Moringa leaves with various concentrations (0.1%, 0.15%, 0.2%, and 0.25%). Several test conducted to get physical and chemical characteristics include enzyme activity test, protein content, amino acid test, water content, and chroma. The results obtained showed that the hydrolysate from overripe tempeh that have been fermented for 5 days with the addition of 0.25% Moringa oleifera leaves purified enzymes has the highest amino acids and low protein content. The color test showed a significant difference in the values of a * and b * due to the treatment between hydrolyzed samples. The value of lightness of overripe tempeh hydrolysate for with 5 days fermentation time and an addition of 0.25% enzymes indicates a significant difference with the control sample. The moisture content obtained in this study have greater value than SNI standards. Moringa oleifera leaves purified protease enzymes purified through the stages of dialysis reach their optimum conditions at 50oC and pH 7.
References
Affandi, D.R., S. Handajani, dan R. Utami. 2010. Kajian Kandungan Protein, Senyawa Antinutrisi, Aktivitas Antioksidan, Dan Sifat Sensoris Tempe Koro Babi (Vicia Faba L.) Dengan Variasi Pengecilan Ukuran. Jurnal Teknologi Hasil Pertanian 3(2): 77–86.
Agustina, Rizki Karunianti, Fillah Fithra Dieny, Ninik Rustanti, Gemala Anjani, and Diana Nur Afifah. 2018. Antioxidant Activity and Soluble Protein Content of Tempeh Gembus Hydrolysate. Hiroshima Journal of Medical Sciences 67 (Special issue): 1–7.
Arnata, I Wayan, Dwi Setyaningsih, and Nur Richana. 2015. Produksi Bioetanol dari Hidrolisat Asam Tepung Ubi Kayu dengan Kultur Campuran Trichoderma viride dan Saccharomyces cerevisiae (Ethanol Production from Acid Hydrolysate Cassava Flour with Mixed Culture Trichoderma viride and Saccharomyces cerevisiae). Jurnal Agritech 35(04): 396.
Aryani, Titin, Fitria Siswi Utami, dan Isnin M Aulia Ulfah. 2016. Pengaruh Lama Penyimpanan Terhadap Kerusakan Asam Lemak Omega-3 pada Air Susu Ibu (ASI). KESMAS. Vol. 10.
Badan Standarisasi Nasional. 2009. Tepung Terigu. Badan Stardisasi Nasional.SNI 3751:2009
Banik, Swarnali, Shrutidhara Biswas, dan Srabani Karmakar. 2018. Extraction, Purification, and Activity of Protease from the Leaves of Moringa Oleifera. F1000Research, 7(1151), 1151.
Barragan, L.A.P., J.J.B. Figueora, L.V. Rodriguez-Duran, C.N.A. Gonzalez, dan C. Hennigs. 2016. “Chapter 7: Fermentative Production Methods.” In Biotransformation of Agricultural Waste and by Product, 189–215.
Bernadeta, Puji Ardiningsih, dan Imelda H. Silalahi. 2012. Penentuan Kondisi Optimum Hidrolisat Protein Dari Limbah Ikan Ekor Kuning (Caesio cuning) Berdasarkan Karakteristik Organoleptik. Jurnal Kimia Khatulistiwa 1(1): 26–30.
Cupp-Enyard, Carrie, dan Sigma Aldrich. 2008. Sigma’s Non-Specific Protease Activity Assay - Casein as a Substrate. Journal of Visualized Experiments, no. 19 (September).
Duong-Ly, Krisna C., dan Sandra B. Gabelli. 2014. Salting out of Proteins Using Ammonium Sulfate Precipitation. In Methods in Enzymology, 541:85–94. Academic Press Inc.
Fahroji, dan Hendri. 2016. Kinerja Beberapa Tipe Moisture Meter Dalam Penentuan Kadar Air Padi. Jurnal Lahan Suboptimal 5(1): 62–70.
Fathimah, Azmy Nahdhiyati, dan Agustina Krisna Wardani. 2014. Ekstraksi Dan Karakterisasi Enzim Protease Dari Daun Kelor (Moringa oleifera Lamk.). Jurnal Teknologi Pertanian 15 (3): 191–200.
Gunawan-Puteri, Maria Dewi Puspitasari Tirtaningtya, Tia Raissha Hassanein, Elisabeth Kartika Prabawati, Christofora Hanny Wijaya, dan Anthony N. Mutukumira. 2015. Sensory Characteristics of Seasoning Powders from Overripe Tempeh, a Solid State Fermented Soybean. Procedia Chemistry. 14:263-269
Hasniar, Muh. Rais, dan Ratnawaty Fadilah. 2019. Analysis of Nutrition Content and Organoleptic Test in Tempe. Jurnal Pendidikan Teknologi Pertanian 5:189–200.
Male, Kasmir, Siti Nuryanti, dan Sitti Rahmawati. 2014. Ekstrak Enzim Protease dari Daun Palado (Agave angustifolia) dan Pemanfaatannya dalam Proses Pembuatan Virgin Coconut Oil.” Jurnal Akademika Kimia 3(3): 111–20.
Mohan, R., Sivakumar, V., Rangasamy, T., dan Muralidharan, C. 2016. Optimisation of bromelain enzyme extraction from pineapple (Ananas comosus) and application in process industry. American Journal of Biochemistry and Biotechnology, 12(3): 188-195.
Mukhoyaroh dan Hanifah. 2015. Pengaruh Jenis Kedelai, Waktu Dan Suhu Pemeraman Terhadap Kandungan Protein Tempe Kedelai. Florea: Jurnal Biologi dan Pembelajarannya 2(2): 47–51.
Mulyana, Wahono Hadi Susanto, dan Indria Purwantiningrum. 2014. The Effect of the Ratio of Over Fermented Tempeh Flour to Tapioca and Level of Water Addition on the Characteristics of Over Ferm. Jurnal Pangan Dan Agroindustri 2(4): 113–20.
Muzdalifah, D., Z. A. Athaillah, W. Nugrahani, dan A. F. Devi. 2017. Colour and PH Changes of Tempe during Extended Fermentation. In AIP Conference Proceedings 1083(1):020-036
Noviyanti, Tri, Puji Ardiningsih, dan Winda Rahmalia. 2012. Pengaruh Temperatur Terhadap Aktivitas Enzim Protease Dari Daun Sansakng (Pycnarrhena cauliflora Diels). Jurnal Kimia Khatulistiwa 1(1): 31–34.
Okfrianti, Yenni, Kamsiah, dan Yessy Fitryani. 2011. Pengaruh Penambahan Enzim Protease Tanaman Terhadap Sifat Fisik Dan Organoleptik Daging Sapi. Jurnal Sain Peternakan Indonesia 6(2): 25–36.
Sanjiwani, P., I.N.K. Widjaja, dan N.K. Warditiani. 2014. Analisis Kuantitatif Asam Lemak Tak Jenuh Pada Virgin Coconut Oil (VCO) Yang Dibuat Dengan Penambahan Sari Getah Pepaya (Carica papaya L.). Jurnal Farmasi Udayana 3(2): 32–35.
Sharmila, S., L.J. Rebecca, dan M. Saduzzaman. 2012. Immobilization of Plant Protease Using Calcium Alginate Beads. Journal of Chemical and Pharmaceutical Research. 10 (4): 4484–88.
Sobri, Akhmad, Herpandi Herpandi, dan Susi Lestari. 2018. Uji Pengaruh Suhu Pengeringan Pada Karakteristik Kimia Dan Sensori Kaldu Bubuk Kepala Ikan Gabus (Channa striata). Jurnal FishtecH 6(2): 97–106.
Sudaryatiningsih, Cicik, dan Supyani. 2010. Analisis Kandungan Asam Linoleat Dan Linolenat Tahu Kedelai Dengan Rhizopus oryzae Dan Rhizopus oligosporus Sebagai Koagulan.” Nusantara Bioscience 2 (1): 110–16.
Taslim, D., dan Siregar, T. M. 2022. Aktivitas Inhibisi α-Glukosidase Dan Antioksidan Ekstrak Etanol Srikaya (Annona squamosa L.). Jurnal Teknologi Pangan, 16(2).
Triwibowo, R., M.A.M Andriani, and S. Ariviani. 2016. “Perubahan Biokimiawi Stakiosa Dan Asam Lemak Esensial Pada Tempe Kedelai (Glycine max.) Selama Proses Fermentasi.” Biokteknologi 13 (1): 34–41.
Pathare, Pankaj B., Umezuruike Linus Opara, dan Fahad Al Julanda Al-Said. 2013. Colour Measurement and Analysis in Fresh and Processed Foods: A Review. Food and Bioprocess Technology 6:36-60
Pontual, Emmanuel V, Belany EA Carvalho, Ranilson S Bezerra, Luana CBB Coelho, Thiago H Napoleão, dan Patrícia MG Paiva. 2012. Caseinolytic and Milk-Clotting Activities from Moringa Oleifera Flowers. Food Chemistry 135: 1848–54.
Pramitha, Dewi Ayu Ika, dan Debby Juliadi. 2019. Pengaruh Suhu Terhadap Bilangan Peroksida dan Asam Lemak Bebas pada VCO ( Virgin Coconut Oil ) Hasil Fermentasi. Cakra Kimia 7 (2): 149–54.
Purich, Daniel L. 2010. Enzyme Kinetics: Catalysis & Control. Enzyme Kinetics: Catalysis & Control. Elsevier Inc.
Purwaningsih, Indah. 2017. Potensi Enzim Bromelin Sari Buah Nanas (Ananas comosus L.) Dalam Meningkatkan Kadar Protein Pada Tahu. Jurnal Teknologi Laboratorium 6 (1): 2–9.
Utami, Rahayu, Christofora Hanny Wijaya, dan Hanifah Nuryani Lioe. 2016. Taste of Water-Soluble Extracts Obtained from Over-Fermented Tempe. International Journal of Food Properties 19 (9): 2063–73.
Wijayanti, I., Romadhon, R., dan Rianingsih, L.(201). Karakteristik Hidrolisat Protein Ikan Bandeng (Chanos chanos Forsk) dengan Konsentrasi Enzim Bromelin yang Berbeda. SAINTEK PERIKANAN: Indonesian Journal of Fisheries Science and Technology, 11(2): 129-133.
Witono, Yuli, Simon Bambang Widjanarko, Mujianto Mujianto, dan Dessy Tri Rachmawati. 2015. Amino Acids Identification of Over Fermented Tempeh, The Hydrolysate and The Seasoning Product Hydrolysed by Calotropin from Crown Flower (Calotropis gigantea). International Journal on Advanced Science, Engineering and Information Technology.
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